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Immunofluorescent staining of human cell line RT4 shows localization to microtubule organizing center & cell junctions.
Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & cell junctions.
Antibody dilution
1:139
1:51
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA043458
Antibody HPA052894
Standard validation
Approved
Figure description
Immunohistochemical staining of human rectum shows moderate membranous positivity in glandular cells.
Expression
RNA: detected in 30 tissues Protein: detected in 34 cell types
Retrieval
HIER pH6
Antibody dilution
1:900
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA043458
Antibody HPA052894
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY413148)
Target mass (kDa)
33.7
33.7
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA043458
Antibody HPA052894
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
