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Antibody staining overlaps with antibody HPA030426.
Validated
Antibody staining overlaps with antibody HPA023915.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
Immunofluorescent staining of human cell line BJ shows localization to endoplasmic reticulum.
Antibody dilution
1:55
1:167
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA023915
Antibody HPA030426
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human lymph node shows cytoplasmic positivity in a subset of cells.
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells.
Expression
RNA: detected in 19 tissues Protein: detected in 69 cell types
RNA: detected in 19 tissues Protein: detected in 62 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:35
1:275
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA023915
Antibody HPA030426
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY409297)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY409297)
Target mass (kDa)
24.2
24.2
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA023915
Antibody HPA030426
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
