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ENAH

ANTIBODY INFORMATION
 
 

Antibody HPA028448

  

Antibody HPA028696

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA028448   HPA028696  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 6   6  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA028448

  

Antibody HPA028696

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry


Validated

Validated
  

Validated

Validated
  
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA028448

  

Antibody HPA028696

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA028696.		  
Validated

Antibody staining overlaps with antibody HPA028448.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane, cytosol & focal adhesion sites.   Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane, cytosol & focal adhesion sites.  

Antibody dilution

 1:100   1:20  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA028448

  

Antibody HPA028696

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 

 

  
 

 

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  

 

  
 

  

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA028696 across 74 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA028448 across 74 cell types.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in basal glandular cells.   Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in basal glandular cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 72 cell types		   RNA: detected in 36 tissues
Protein: detected in 63 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:500   1:300  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA028448

  

Antibody HPA028696

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 87.4, 66.5, 63.9   66.5, 63.9  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA028448

  

Antibody HPA028696

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA028448

  

Antibody HPA028696

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 104   91  

Antigen sequence

 
STSTPEPTRKPWERTNTMNGSKSPVISRRDSPRKNQIVFDNRSYDSLHRP
KSTPLSQPSANGVQTEGLDYDRLKQDILDEMRKELTKLKEELIDAIRQEL
SKSN
  
RQERQERERLERLERERQERERQEQLEREQLEWERERRISSAAAPASVET
PLNSVLGDSSASEPGLQAASQPAETPSQQGIVLGPLAPPPP
 

Matching transcripts

 ENAH-006 - ENSP00000355809 [100%]
ENAH-001 - ENSP00000355808 [80%]
ENAH-011 - ENSP00000489607 [80%]
  ENAH-001 - ENSP00000355808 [100%]
ENAH-006 - ENSP00000355809 [100%]
 

Other gene match

    
ANTIGEN VIEW
ENAH-001
ENAH-006
ENAH-011
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.