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PPP4R1

ANTIBODY INFORMATION
 
 

Antibody HPA040905

  

Antibody HPA041089

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA040905   HPA041089  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 8   8  

References

   2  

VALIDATION SUMMARY
 
 

Antibody HPA040905

  

Antibody HPA041089

  
Immunocytochemistry

Approved    
Immunohistochemistry

Uncertain   Uncertain  
Western blot


Validated
   Supported  
Protein array

Approved   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA040905

  

Antibody HPA041089

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.    

Antibody dilution

 1:20    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA040905

  

Antibody HPA041089

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 74 cell types		   RNA: detected in 37 tissues
Protein: detected in 74 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:35   1:400  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA040905

  

Antibody HPA041089

  

Formal validation: Genetic
Validated

Downregulation visible in both siRNA lanes		    

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		    

Target mass (kDa)

 107, 105.2    

Loading control

 Total protein image    

Antibody dilution

 1:58    
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 107, 105.2   107, 105.2  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA040905

  

Antibody HPA041089

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA040905

  

Antibody HPA041089

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 77   76  

Antigen sequence

 
PLDSSLLCTLSSESHQEAASNENDKKPGNYKSMLRPEVGTTSQDSALLDQ
ELYNSFHFWRTPLPEIDLDIELEQNSG
  
LDAHEETISIEKRSDLQDELDINELPNCKINQEDSVPLISDAVENMDSTL
HYIHSDSDLSNNSSFSPDEERRTKVQ
 

Matching transcripts

 PPP4R1-001 - ENSP00000383402 [100%]
PPP4R1-002 - ENSP00000383401 [100%]
  PPP4R1-001 - ENSP00000383402 [100%]
PPP4R1-002 - ENSP00000383401 [100%]
 

Other gene match

    
ANTIGEN VIEW
PPP4R1-001
PPP4R1-002

RELEVANT PUBLICATIONS
 
 

Antibody HPA040905

  

Antibody HPA041089

  

1.

   PP4R1 accelerates cell growth and proliferation in HepG2 hepatocellular carcinoma
Wu G et al
Onco Targets Ther 2015;8:2067-74
  

2.

   Lentivirus-Mediated Short-Hairpin RNA Targeting Protein Phosphatase 4 Regulatory Subunit 1 Inhibits Growth in Breast Cancer
Qi Y et al
J Breast Cancer 2015;18(3):218-24
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.