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PIEZO2

ANTIBODY INFORMATION
 
 

Antibody HPA015986

  

Antibody HPA031974

  

Antibody HPA031975

  

Antibody HPA040616

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA015986   HPA031974   HPA031975   HPA040616  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

        

Released in version

 4   6   16   10  

References

   1      

VALIDATION SUMMARY
 
 

Antibody HPA015986

  

Antibody HPA031974

  

Antibody HPA031975

  

Antibody HPA040616

  
Immunocytochemistry

    Approved    
Immunohistochemistry

Approved   Approved     Approved  
Western blot

Uncertain   Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA015986

  

Antibody HPA031974

  

Antibody HPA031975

  

Antibody HPA040616

  

Standard validation

     Approved    

       

Figure description

     Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm & vesicles.    

Antibody dilution

     1:5    

Literature conformity

     The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA015986

  

Antibody HPA031974

  

Antibody HPA031975

  

Antibody HPA040616

  

Standard validation

 Approved   Approved     Approved  

       

Figure description

 Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human gall bladder shows strong membrane and cytoplasmic positivity in glandular cells.     Immunohistochemical staining of human breast shows strong cytoplasmic and membranous positivity in glandular cells.  

Expression

 RNA: detected in 27 tissues
Protein: detected in 64 cell types		   RNA: detected in 27 tissues
Protein: detected in 56 cell types		     RNA: detected in 27 tissues
Protein: detected in 28 cell types		  

Retrieval

 HIER pH6   HIER pH6     HIER pH6  

Antibody dilution

 1:80   1:1300     1:375  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.     No avaliable gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.     Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA015986

  

Antibody HPA031974

  

Antibody HPA031975

  

Antibody HPA040616

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

       

Figure description

     Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 320.9, 318.1, 312.6, 311.1, 80.8   320.9, 318.1, 312.6, 311.1   320.9, 318.1, 312.6, 311.1, 20.5   320.9, 318.1, 312.6, 311.1  

Antibody dilution

 1:250   1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA015986

  

Antibody HPA031974

  

Antibody HPA031975

  

Antibody HPA040616

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000   1:500   1:6000  

ANTIGEN INFORMATION
 
 

Antibody HPA015986

  

Antibody HPA031974

  

Antibody HPA031975

  

Antibody HPA040616

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 123   67   42   33  

Antigen sequence

 
ESEKPIFTMSAQQSQLKVMDQQSFNKFIQAFSRDTGAMQFLENYEKEDIT
VAELEGNSNSLWTISPPSKQKMIHELLDPNSSFSVVFSWSIQRNLSLGAK
SEIATDKLSFPLKNITRKNIAKM
  
KPVTDEAAQSNPEFENEELAEGEKIDSEEALIYEEDFNGGDGVEGELEES
TKLKMFRRLASVASKLK
  
QFQFFQEAVPPNDYYARLFGIKSVIQTDCSSTWKIIVNPDLS
  
FEDENKAAVRIMAGDNVEICMNLDAASFSQHNP
 

Matching transcripts

 PIEZO2-007 - ENSP00000303316 [100%]
PIEZO2-003 - ENSP00000421377 [97%]
PIEZO2-006 - ENSP00000463094 [97%]
PIEZO2-011 - ENSP00000443129 [97%]
PIEZO2-201 - ENSP00000372900 [97%]
  PIEZO2-003 - ENSP00000421377 [100%]
PIEZO2-006 - ENSP00000463094 [100%]
PIEZO2-007 - ENSP00000303316 [100%]
PIEZO2-201 - ENSP00000372900 [100%]
  PIEZO2-003 - ENSP00000421377 [100%]
PIEZO2-006 - ENSP00000463094 [100%]
PIEZO2-007 - ENSP00000303316 [100%]
PIEZO2-013 - ENSP00000463589 [100%]
PIEZO2-201 - ENSP00000372900 [100%]
  PIEZO2-003 - ENSP00000421377 [100%]
PIEZO2-006 - ENSP00000463094 [100%]
PIEZO2-007 - ENSP00000303316 [100%]
PIEZO2-201 - ENSP00000372900 [100%]
 

Other gene match

        
ANTIGEN VIEW
PIEZO2-003
PIEZO2-006
PIEZO2-007
PIEZO2-011
PIEZO2-013
PIEZO2-201

RELEVANT PUBLICATIONS
 
 

Antibody HPA015986

  

Antibody HPA031974

  

Antibody HPA031975

  

Antibody HPA040616

  

1.

   Tuning Piezo ion channels to detect molecular-scale movements relevant for fine touch
Poole K et al
Nat Commun 2014;5:3520
      
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.