Fields »
ABOUT
HELP
BLOG
APOOL

ANTIBODY INFORMATION
 
 

Antibody HPA000612

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA000612  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 1  

References

 3  

VALIDATION SUMMARY
 
 

Antibody HPA000612

  
Immunocytochemistry

Supported  
Immunohistochemistry

Approved  
Western blot

Supported  
Protein array

Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA000612

  

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.  

Antibody dilution

 1:167  

Literature conformity

 The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA000612

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 41 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:750  

Literature conformity

 No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA000612

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

 

Figure description

 Lane 1: Marker [kDa] 207, 110, 79, 49, 32, 25, 17.1
Lane 2: RT4
Lane 3: EFO-21
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 29.2  

Antibody dilution

 1:500  

PROTEIN ARRAY
 
 

Antibody HPA000612

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:2000  

ANTIGEN INFORMATION
 
 

Antibody HPA000612

  

Antigen

 Recombinant protein fragment  

Length (aa)

 116  

Antigen sequence

 
ATLGATVCYPVQSVIIAKVTAKKVYATSQQIFGAVKSLWTKSSKEESLPK
PKEKTKLGSSSEIEVPAKTTHVLKHSVPLPTELSSEAKTKSESTSGATQF
MPDPKLMDHGQSHPED
 

Matching transcripts

 APOOL-001 - ENSP00000362268 [100%]
APOOL-201 - ENSP00000481770 [100%]
 

Other gene match

  
ANTIGEN VIEW
APOOL-001
APOOL-201

RELEVANT PUBLICATIONS
 
 

Antibody HPA000612

  

1.

 Systematically generated antibodies against human gene products: high throughput screening on sections from the rat nervous system
Mulder J et al
Neuroscience 2007;146(4):1689-703
  

2.

 Tissue profiling of the mammalian central nervous system using human antibody-based proteomics
Mulder J et al
Mol Cell Proteomics 2009;8(7):1612-22
  

3.

 APOOL is a cardiolipin-binding constituent of the Mitofilin/MINOS protein complex determining cristae morphology in mammalian mitochondria
Weber TA et al
PLoS One 2013;8(5):e63683
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.