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AK9

ANTIBODY INFORMATION
 
 

Antibody HPA030804

  

Antibody HPA030805

  

Antibody HPA031779

  

Antibody HPA031781

  

Antibody HPA036324

  

Antibody HPA036711

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA030804   HPA030805   HPA031779   HPA031781   HPA036324   HPA036711  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb   pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

            

Released in version

 12   12   6   12   12   12  

References

            

VALIDATION SUMMARY
 
 

Antibody HPA030804

  

Antibody HPA030805

  

Antibody HPA031779

  

Antibody HPA031781

  

Antibody HPA036324

  

Antibody HPA036711

  
Immunocytochemistry


Validated
  

Validated
          
Immunohistochemistry

Approved   Approved   Approved   Approved   Approved   Approved  
Western blot

Supported   Uncertain   Supported   Uncertain   Supported   Uncertain  
Protein array

Supported   Approved   Supported   Approved   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA030804

  

Antibody HPA030805

  

Antibody HPA031779

  

Antibody HPA031781

  

Antibody HPA036324

  

Antibody HPA036711

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA030805.		  
Validated

Antibody staining overlaps with antibody HPA030804.		          
 

Standard validation

 Supported   Supported          

           

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear membrane.   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear membrane.          

Antibody dilution

 1:38   1:185          

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.          

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA030804

  

Antibody HPA030805

  

Antibody HPA031779

  

Antibody HPA031781

  

Antibody HPA036324

  

Antibody HPA036711

  

Standard validation

 Approved   Approved   Approved   Approved   Approved   Approved  

           

Figure description

 Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.   Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.   Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in lymphoid cells outside reaction centra.   Immunohistochemical staining of human placenta shows strong cytoplasmic and membranous positivity in trophoblastic cells.   Immunohistochemical staining of human stomach, upper shows strong nuclear positivity in glandular cells.   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 76 cell types		   RNA: detected in 36 tissues
Protein: detected in 73 cell types		   RNA: detected in 36 tissues
Protein: detected in 51 cell types		   RNA: detected in 36 tissues
Protein: detected in 76 cell types		   RNA: detected in 36 tissues
Protein: detected in 77 cell types		   RNA: detected in 36 tissues
Protein: detected in 62 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:100   1:600   1:150   1:250   1:100   1:80  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA030804

  

Antibody HPA030805

  

Antibody HPA031779

  

Antibody HPA031781

  

Antibody HPA036324

  

Antibody HPA036711

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

           

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 221.4, 84.9, 48.5   221.4, 84.9   221.4, 87.2, 36.4   221.4, 87.2, 36.4   221.4, 39, 25.3   221.4, 39  

Antibody dilution

 1:250   1:250   1:250   1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA030804

  

Antibody HPA030805

  

Antibody HPA031779

  

Antibody HPA031781

  

Antibody HPA036324

  

Antibody HPA036711

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

           

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000   1:3000   1:3000   1:3000   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA030804

  

Antibody HPA030805

  

Antibody HPA031779

  

Antibody HPA031781

  

Antibody HPA036324

  

Antibody HPA036711

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 82   88   91   103   68   86  

Antigen sequence

 
VMAEHNPQYLIELNGNKPAEELFMIVMDRLKYLNLKRAAILTKLQGAEEE
INDTMENDELFRTLASYKLIAPRYRWQRSKWG
  
VVTMIEETIKMSQDINFEQPYEKHAEILQEVLGEVMEENKDRFPGAPKYG
GWIVDNCPIVKELWMALIKKGIIPDLVIYLSDTENNGK
  
YCPVTYKDGNQRYEALVPGSINYALEYHNRIYICENKEKLQKFLRSPLKY
WEQKLPHKLPPLREPILLTSLPLPGYLEQGIATSLIKAMNA
  
CIDKLCITPQELLSRLGEFEQFCPVSLAESQELFDCSATDSLEFAAEFRG
HYYKMSSQEKLNKFLENPELYVPPLAPHPLPSADMIPKRLTLSELKSRFP
KCA
  
SEWWLKEPIRSTGFILDGFPRYPEEAQFLGDRGFFPDAAVFIQVDDQDIF
DRLLPAQIEKWKLKQKKK
  
ERKRHLGDTKHFCPVVLKENFILQPGNTEEAAKYREKIYYFSSAEAKEKF
LEHPEDYVAHEEPLKAPPLRICLVGPQGSGKTMCGR
 

Matching transcripts

 AK9-006 - ENSP00000357944 [100%]
AK9-007 - ENSP00000285397 [100%]
AK9-201 - ENSP00000410186 [100%]
  AK9-006 - ENSP00000357944 [100%]
AK9-201 - ENSP00000410186 [100%]
  AK9-003 - ENSP00000418771 [100%]
AK9-004 - ENSP00000419758 [100%]
AK9-201 - ENSP00000410186 [100%]
  AK9-003 - ENSP00000418771 [100%]
AK9-201 - ENSP00000410186 [100%]
AK9-004 - ENSP00000419758 [90%]
  AK9-001 - ENSP00000347431 [100%]
AK9-002 - ENSP00000418670 [100%]
AK9-201 - ENSP00000410186 [100%]
  AK9-001 - ENSP00000347431 [100%]
AK9-201 - ENSP00000410186 [100%]
 

Other gene match

            
ANTIGEN VIEW
AK9-001
AK9-002
AK9-003
AK9-004
AK9-006
AK9-007
AK9-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.