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ANTIBODY INFORMATION
 
 

Antibody HPA059588

  

Antibody HPA064962

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA059588   HPA064962  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 14   14  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA059588

  

Antibody HPA064962

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

  Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA059588

  

Antibody HPA064962

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA064962.		  
Validated

Antibody staining overlaps with antibody HPA059588.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.  

Antibody dilution

 1:8   1:45  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA059588

  

Antibody HPA064962

  

Standard validation

   Approved  

   

Figure description

   Immunohistochemical staining of human appendix shows cytoplasmic positivity in leukocytes.  

Expression

   RNA: detected in 28 tissues
Protein: detected in 8 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:250  

Literature conformity

   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA059588

  

Antibody HPA064962

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 14.1, 8.4   14.1  

Antibody dilution

 1:80   1:150  

PROTEIN ARRAY
 
 

Antibody HPA059588

  

Antibody HPA064962

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:1800  

ANTIGEN INFORMATION
 
 

Antibody HPA059588

  

Antibody HPA064962

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 30   43  

Antigen sequence

 
DSSDSSFSRSPPPGKQDSSDDVRRVQRREK
  
TEELKYFTSVLNSHEPLCSVLAASTPSPPEVVYSAHAFHQPHV
 

Matching transcripts

 BATF-001 - ENSP00000286639 [100%]
BATF-003 - ENSP00000450486 [100%]
  BATF-001 - ENSP00000286639 [100%]
 

Other gene match

    
ANTIGEN VIEW
BATF-001
BATF-003
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.