CCT8 - Antibodies - The Human Protein Atlas

We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.

CCT8

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA018520

Antibody HPA021051

Antibody HPA029426

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA018520

HPA021051

HPA029426

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA018520

Antibody HPA021051

Antibody HPA029426

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA018520

Antibody HPA021051

Antibody HPA029426

Standard validation

Approved

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.

Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & intermediate filaments.

Antibody dilution

1:10

1:100

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA018520

Antibody HPA021051

Antibody HPA029426

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA029426 across 74 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA021051 across 74 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.

Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.

Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 71 cell types

RNA: detected in 37 tissues
Protein: detected in 58 cell types

Retrieval

HIER pH6

Antibody dilution

1:400

1:50

1:650

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA018520

Antibody HPA021051

Antibody HPA029426

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

59.6, 57.6, 51.6

59.6, 57.6, 51.6, 35.4

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA018520

Antibody HPA021051

Antibody HPA029426

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:500

1:10000

ANTIGEN INFORMATION

Antibody HPA018520

Antibody HPA021051

Antibody HPA029426

Antigen

Recombinant protein fragment

Length (aa)

145

122

Antigen sequence

LPNLVCCSAKNLRDIDEVSSLLRTSIMSKQYGNEVFLAKLIAQACVSIFP
DSGHFNVDNIRVCKILGSGISSSSVLHGMVFKKETEGDVT

KVLTRDKRLVPGGGATEIELAKQITSYGETCPGLEQYAIKKFAEAFEAIP
RALAENSGVKANEVISKLYAVHQEGNKNVGLDIEAEVPAVKDMLEAGILD
TYLGKYWAIKLATNAAVTVLRVDQIIMAKPAGGPKPPSGKKDWDD

VYSCPFDGMITETKGTVLIKTAEELMNFSKGEENLMDAQVKAIADTGANV
VVTGGKVADMALHYANKYNIMLVRLNSKWDLRRLCKTVGATALPRLTPPV
LEEMGHCDSVYLSEVGDTQVVV

Matching transcripts

CCT8-003 - ENSP00000286788 [100%]
CCT8-012 - ENSP00000486921 [100%]
CCT8-201 - ENSP00000442730 [100%]

CCT8-003 - ENSP00000286788 [100%]
CCT8-012 - ENSP00000486921 [100%]
CCT8-201 - ENSP00000442730 [100%]
CCT8-006 - ENSP00000416832 [89%]

Other gene match

ANTIGEN VIEW

CCT8-003	CCT8-006	CCT8-012	CCT8-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.