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SH3RF2

ANTIBODY INFORMATION
 
 

Antibody HPA035903

  

Antibody HPA058119

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA035903   HPA058119  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   13  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA035903

  

Antibody HPA058119

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

  Uncertain  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA035903

  

Antibody HPA058119

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA058119.		  
Validated

Antibody staining overlaps with antibody HPA035903.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm.  

Antibody dilution

 1:96   1:60  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA035903

  

Antibody HPA058119

  

Standard validation

   Uncertain  

   

Figure description

   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in a subset of cells in seminiferous ducts.  

Expression

   RNA: detected in 29 tissues
Protein: detected in 44 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:800  

Literature conformity

   No avaliable gene/protein characterization data.  

RNA consistency

   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA035903

  

Antibody HPA058119

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 79.3   79.3  

Antibody dilution

 1:250   1:200  

PROTEIN ARRAY
 
 

Antibody HPA035903

  

Antibody HPA058119

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:2400  

ANTIGEN INFORMATION
 
 

Antibody HPA035903

  

Antibody HPA058119

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 83   85  

Antigen sequence

 
NYSPPPTKHYTSHPTSGKPEQPATLKASQPEAASLGPEMTVLFAHRSGCH
SGQQTDLRRKSALAKATTLVSTASGTQTVFPSK
  
QGSLRKGRSSMRKNGSLQRPLQSGIPTLVVGSLRRSPTMVLRPQQFQFYQ
PQGIPSSPSAVVVEMGSKPALTGEPALTCISRGSE
 

Matching transcripts

 SH3RF2-001 - ENSP00000352028 [99%]
SH3RF2-002 - ENSP00000424497 [99%]
  SH3RF2-001 - ENSP00000352028 [100%]
SH3RF2-002 - ENSP00000424497 [100%]
 

Other gene match

    
ANTIGEN VIEW
SH3RF2-001
SH3RF2-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.