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ATAD2

ANTIBODY INFORMATION
 
 

Antibody HPA029424

  

Antibody CAB056158

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA029424   AMAb90541  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 6   10  

References

 2    

VALIDATION SUMMARY
 
 

Antibody HPA029424

  

Antibody CAB056158

  
Immunocytochemistry


Validated
    
Immunohistochemistry

Supported   Supported  
Western blot


Validated
   Supported  
Protein array

Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA029424

  

Antibody CAB056158

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by both siRNA:s.		    

 

Relative fluorescence intensity (RFI)

    

Figure description

 Nuclear region of segmented cells in 10x-images    

Antibody dilution

 1:70    
 

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.    

Antibody dilution

 1:50    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA029424

  

Antibody CAB056158

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.   Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.  

Expression

 RNA: detected in 35 tissues
Protein: detected in 58 cell types		   RNA: detected in 35 tissues
Protein: detected in 37 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:1000  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA029424

  

Antibody CAB056158

  

Formal validation: Genetic
Validated

Downregulation visible in one of two siRNA lanes		    

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		    

Target mass (kDa)

 158.6, 80.6    

Loading control

 Total protein image    

Antibody dilution

 1:156    
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 158.6, 80.6   158.6, 80.6  

Antibody dilution

 1:250   1:1000  

PROTEIN ARRAY
 
 

Antibody HPA029424

  

Antibody CAB056158

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA029424

  

Antibody CAB056158

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 114    

Antigen sequence

 
TAYAIIKEELDEDFEQLCEEIQESRKKRGCSSSKYAPSYYHVMPKQNSTL
VGDKRSDPEQNEKLKTPSTPVACSTPAQLKRKIRKKSNWYLGTIKKRRKI
SQAKDDSQNAIDHK
  

											 
																				

																		

										
											
Matching transcripts

												
																					ATAD2-001 - ENSP00000287394 [100%] 
ATAD2-002 - ENSP00000429213 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ATAD2-001
ATAD2-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA029424

												
										 
																				
											
											
											
Antibody CAB056158

												
										 
																		

																	

										
											
1.

												
																						Integrated genomic analysis of the 8q24 amplification in endometrial cancers identifies ATAD2 as essential to MYC-dependent cancers

	Raeder MB et al

	PLoS One 2013;8(2):e54873

			
											 
																						
											 
																				

																		

										
											
2.

												
																						ATAD2 is highly expressed in ovarian carcinomas and indicates poor prognosis

	Wan WN et al

	Asian Pac J Cancer Prev 2014;15(6):2777-83

			
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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by the Knut & Alice Wallenberg foundation.