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SHCBP1L

ANTIBODY INFORMATION
 
 

Antibody HPA036124

  

Antibody HPA036125

  

Antibody HPA064821

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA036124   HPA036125   HPA064821  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 12   7   13  

References

 1      

VALIDATION SUMMARY
 
 

Antibody HPA036124

  

Antibody HPA036125

  

Antibody HPA064821

  
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA036124

  

Antibody HPA036125

  

Antibody HPA064821

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in subset of cells in seminiferus ducts.  

Expression

 RNA: detected in 1 tissues
Protein: detected in 19 cell types		   RNA: detected in 1 tissues
Protein: detected in 5 cell types		   RNA: detected in 1 tissues
Protein: detected in 10 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:300   1:1000   1:25000  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA036124

  

Antibody HPA036125

  

Antibody HPA064821

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

      

Target mass (kDa)

 80.5, 72.6   80.5, 72.6   80.5, 72.6  

Antibody dilution

 1:250   1:250   1:1170  

PROTEIN ARRAY
 
 

Antibody HPA036124

  

Antibody HPA036125

  

Antibody HPA064821

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000   1:14100  

ANTIGEN INFORMATION
 
 

Antibody HPA036124

  

Antibody HPA036125

  

Antibody HPA064821

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 99   86   78  

Antigen sequence

 
CVSRMRGMWRDEKVSLYCDEVLQDCKAEDADEVMGKYLSEKLKLKDKWLG
VWKTNPSVFFVKYEEASIPFVGILVEVTCEPYQDSSSRFKVTVSVAEPF
  
FPGEYQAANLALLTDDIIIKGVGKREEIMITSEPSRDSFVVSKADNVKLM
HLSLIQQGTVDGIVVVESGHMTLENCILKCEGTGVC
  
TLRGVNMKVLPAPKLKMTNNHIYSNKGYGVSILQPMEQFFIVAEEALNKR
ASSGDKKDDKMLFKVMQNLNLEMNNNKI
 

Matching transcripts

 SHCBP1L-001 - ENSP00000356518 [100%]
SHCBP1L-201 - ENSP00000397308 [100%]
  SHCBP1L-001 - ENSP00000356518 [100%]
SHCBP1L-201 - ENSP00000397308 [100%]
  SHCBP1L-001 - ENSP00000356518 [99%]
SHCBP1L-201 - ENSP00000397308 [99%]
 

Other gene match

      
ANTIGEN VIEW
SHCBP1L-001
SHCBP1L-201

RELEVANT PUBLICATIONS
 
 

Antibody HPA036124

  

Antibody HPA036125

  

Antibody HPA064821

  

1.

 The human testis-specific proteome defined by transcriptomics and antibody-based profiling
Djureinovic D et al
Mol Hum Reprod 2014;20(6):476-88
      
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.