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CPT2

ANTIBODY INFORMATION
 
 

Antibody HPA028201

  

Antibody HPA028202

  

Antibody HPA028214

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA028201   HPA028202   HPA028214  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 6   6   6  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA028201

  

Antibody HPA028202

  

Antibody HPA028214

  
Immunocytochemistry

    Supported  
Immunohistochemistry


Validated
  

Validated

Validated
   Supported  
Western blot

Supported   Uncertain   Supported  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA028201

  

Antibody HPA028202

  

Antibody HPA028214

  

Standard validation

     Supported  

     

Figure description

     Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, nucleoli & mitochondria.  

Antibody dilution

     1:20  

Literature conformity

     The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA028201

  

Antibody HPA028202

  

Antibody HPA028214

  

Formal validation: Orthogonal

  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		    

 
 

 

    

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.    
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		    

 

  
 

    

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA028202 across 75 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA028201 across 75 cell types.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 48 cell types		   RNA: detected in 37 tissues
Protein: detected in 53 cell types		   RNA: detected in 37 tissues
Protein: detected in 36 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:13   1:13   1:35  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA028201

  

Antibody HPA028202

  

Antibody HPA028214

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 73.8   73.8   73.8  

Antibody dilution

 1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA028201

  

Antibody HPA028202

  

Antibody HPA028214

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA028201

  

Antibody HPA028202

  

Antibody HPA028214

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 79   99   85  

Antigen sequence

 
CKSFENGIGKELHEQLVALDKQNKHTSYISGPWFDMYLSARDSVVLNFNP
FMAFNPDPKSEYNDQLTRATNMTVSAIRF
  
MMVECSKYHGQLTKEAAMGQGFDRHLFALRHLAAAKGIILPELYLDPAYG
QINHNVLSTSTLSSPAVNLGGFAPVVSDGFGVGYAVHDNWIGCNVSSYP
  
DTITFKRLIRFVPSSLSWYGAYLVNAYPLDMSQYFRLFNSTRLPKPSRDE
LFTDDKARHLLVLRKGNFYIFDVLDQDGNIVSPSE
 

Matching transcripts

 CPT2-001 - ENSP00000360541 [100%]
  CPT2-001 - ENSP00000360541 [100%]
  CPT2-001 - ENSP00000360541 [100%]
 

Other gene match

      
ANTIGEN VIEW
CPT2-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.