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Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.
Expression
RNA: detected in 32 tissues Protein: detected in 35 cell types
Retrieval
HIER pH6
Antibody dilution
1:25
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA047136
Antibody HPA060203
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY406322)
Target mass (kDa)
25.6, 23.6, 7
25.6, 23.6
Antibody dilution
1:125
1:370
PROTEIN ARRAY
Antibody HPA047136
Antibody HPA060203
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:950
1:4450
ANTIGEN INFORMATION
Antibody HPA047136
Antibody HPA060203
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
51
35
Antigen sequence
TLRGVGPCRKDGLQGQLEEMTELESPKCKRQENEQLLDQNQEIRASQRSW
V
