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ANTIBODY INFORMATION
 
 

Antibody HPA062641

  

Antibody HPA069323

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA062641   HPA069323  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 16   16  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA062641

  

Antibody HPA069323

  
Immunocytochemistry

Approved   Approved  
Immunohistochemistry

Supported    
Western blot

Uncertain   Uncertain  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA062641

  

Antibody HPA069323

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus & microtubule organizing center.   Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus.  

Antibody dilution

 1:200   1:47  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA062641

  

Antibody HPA069323

  

Standard validation

 Supported    

   

Figure description

 Immunohistochemical staining of human lactating breast shows moderate cytoplasmic positivity in glandular cells.    

Expression

 RNA: detected in 18 tissues
Protein: detected in 2 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:30000    

Literature conformity

 Consistent with extensive gene/protein characterization data.    

RNA consistency

 No internal RNA expression data available for correlation.    

WESTERN BLOT
 
 

Antibody HPA062641

  

Antibody HPA069323

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 146.4   146.4  

Antibody dilution

 1:655   1:110  

PROTEIN ARRAY
 
 

Antibody HPA062641

  

Antibody HPA069323

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:11750   1:1900  

ANTIGEN INFORMATION
 
 

Antibody HPA062641

  

Antibody HPA069323

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 92   60  

Antigen sequence

 
GFVCFISADDVPGSNITGICNDETVFAKDKVTCVGHIIGAVVADTPEHTQ
RAAQGVKITYEELPAIITIEDAIKNNSFYGPELKIEKGDLKK
  
KRLEPYKKKNPSGSWEDWVTAAYMDTVSLSATGFYRTPNLGYSFETNSGN
PFHYFSYGVA
 

Matching transcripts

 XDH-001 - ENSP00000368727 [100%]
  XDH-001 - ENSP00000368727 [100%]
 

Other gene match

    
ANTIGEN VIEW
XDH-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.