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EPB41

ANTIBODY INFORMATION
 
 

Antibody HPA028076

  

Antibody HPA028412

  

Antibody HPA028414

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA028076   HPA028412   HPA028414  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 16   14   6  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA028076

  

Antibody HPA028412

  

Antibody HPA028414

  
Immunocytochemistry


Validated
  

Validated
    
Immunohistochemistry

   

Validated
  
Western blot

Supported   Supported   Supported  
Protein array

Approved   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA028076

  

Antibody HPA028412

  

Antibody HPA028414

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA028412.		  
Validated

Antibody staining overlaps with antibody HPA028076.		    
 

Standard validation

 Approved   Supported    

     

Figure description

 Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol & cell junctions.   Immunofluorescent staining of human cell line HEK 293 shows localization to plasma membrane, cytosol & cell junctions.    

Antibody dilution

 1:24   1:89    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA028076

  

Antibody HPA028412

  

Antibody HPA028414

  

Formal validation: Orthogonal

    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

   
 

 

  

Figure description

     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

     Supported  

     

Figure description

     Immunohistochemical staining of human stomach shows strong positivity in erythrocytes.  

Expression

     RNA: detected in 37 tissues
Protein: detected in 0 cell types		  

Retrieval

     HIER pH6  

Antibody dilution

     1:1500  

Literature conformity

     Consistent with extensive gene/protein characterization data.  

RNA consistency

     Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA028076

  

Antibody HPA028412

  

Antibody HPA028414

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 97, 86.6, 72, 69.9, 66.4   97, 86.6, 81.2   97, 86.6, 81.2, 72, 69.9, 66.4  

Antibody dilution

 1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA028076

  

Antibody HPA028412

  

Antibody HPA028414

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:30000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA028076

  

Antibody HPA028412

  

Antibody HPA028414

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 71   93   91  

Antigen sequence

 
ESVPEPRPSEWDKRLSTHSPFRTLNINGQIPTGEGPPLVKTQTVTISDNA
NAVKSEIPTKDVPIVHTETKT
  
EKSLVTEAENSQHQQKEEGEEAINSGQQEPQQEESCQTAAEGDNWCEQKL
KASNGDTPTHEDLTKNKERTSESRGLSRLFSSFLKRPKSQVSE
  
PAPHFERTASKRASRSLDGAAAVDSADRSPRPTSAPAITQGQVAEGGVLD
ASAKKTVVPKAQKETVKAEVKKEDEPPEQAEPEPTEAWKVE
 

Matching transcripts

 EPB41-001 - ENSP00000362904 [100%]
EPB41-003 - ENSP00000362906 [100%]
EPB41-004 - ENSP00000317597 [100%]
EPB41-005 - ENSP00000290100 [100%]
EPB41-201 - ENSP00000345259 [100%]
EPB41-202 - ENSP00000348397 [100%]
  EPB41-001 - ENSP00000362904 [100%]
EPB41-002 - ENSP00000362903 [100%]
EPB41-005 - ENSP00000290100 [100%]
EPB41-201 - ENSP00000345259 [100%]
  EPB41-001 - ENSP00000362904 [100%]
EPB41-002 - ENSP00000362903 [100%]
EPB41-004 - ENSP00000317597 [100%]
EPB41-201 - ENSP00000345259 [100%]
EPB41-003 - ENSP00000362906 [98%]
EPB41-005 - ENSP00000290100 [98%]
EPB41-202 - ENSP00000348397 [98%]
 

Other gene match

      
ANTIGEN VIEW
EPB41-001
EPB41-002
EPB41-003
EPB41-004
EPB41-005
EPB41-201
EPB41-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.