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Immunohistochemical staining of human bone marrow shows strong nuclear positivity in bone marrow poietic cells.
Expression
RNA: detected in 35 tissues Protein: detected in 50 cell types
Retrieval
HIER pH6
Antibody dilution
1:25
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA004176
Antibody HPA037912
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419764)
Target mass (kDa)
51.8, 48.7, 41.4
51.8, 48.7, 27.8, 27.4
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA004176
Antibody HPA037912
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
