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Immunofluorescent staining of human cell line SH-SY5Y shows localization to vesicles.
Antibody dilution
1:50
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA029298
Antibody HPA069790
Antibody CAB002426
Antibody CAB002921
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human small intestine shows strong membranous positivity in glandular cells.
Immunohistochemical staining of human duodenum shows strong membranous and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human kidney shows strong membranous positivity in cells in tubules.
Expression
RNA: detected in 36 tissues Protein: detected in 13 cell types
RNA: detected in 36 tissues Protein: detected in 25 cell types
RNA: detected in 36 tissues Protein: detected in 18 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:70
1:100
1:500
Literature conformity
No avaliable gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA029298
Antibody HPA069790
Antibody CAB002426
Antibody CAB002921
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407289)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
149.7, 142.4, 83.3, 78.7
149.7, 142.4, 35.5
149.7, 142.4, 83.3, 78.7, 35.5, 33, 15.4
149.7, 142.4, 83.3, 78.7, 35.5, 33, 15.4
Antibody dilution
1:125
1:170
1:500
1:500
PROTEIN ARRAY
Antibody HPA029298
Antibody HPA069790
Antibody CAB002426
Antibody CAB002921
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
