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ANKLE1

ANTIBODY INFORMATION
 
 

Antibody HPA026789

  

Antibody HPA073498

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA026789   HPA073498  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 6   16  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA026789

  

Antibody HPA073498

  
Immunocytochemistry

Approved    
Immunohistochemistry

  Approved  
Western blot

Supported   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA026789

  

Antibody HPA073498

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm & cytosol.    

Antibody dilution

 1:6    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA026789

  

Antibody HPA073498

  

Standard validation

   Approved  

   

Figure description

   Immunohistochemical staining of human bone marrow shows moderate cytoplasmic positivity in hematopoietic cells.  

Expression

   RNA: detected in 12 tissues
Protein: detected in 5 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:1400  

Literature conformity

   No avaliable gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA026789

  

Antibody HPA073498

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 72.4, 69.4   72.4  

Antibody dilution

 1:250   1:1060  

PROTEIN ARRAY
 
 

Antibody HPA026789

  

Antibody HPA073498

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:12700  

ANTIGEN INFORMATION
 
 

Antibody HPA026789

  

Antibody HPA073498

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 126   80  

Antigen sequence

 
GVVKSSFTYLLLDPRETQDLPARAFSLTPAERLQTFIRAIFYVGKGTRAR
PYVHLWEALGHHGRSRKQPHQACPKVRQILDIWASGCGVVSLHCFQHVVA
VEAYTREACIVEALGIQTLTNQKQGH
  
STVSDLELLKGLRALGENPHPITPFTRQLYHQQLEEAQIAPGPEFSGHSL
ELAAALRTGCIPDVQADEDALAQQFEQPDP
 

Matching transcripts

 ANKLE1-001 - ENSP00000468845 [100%]
ANKLE1-003 - ENSP00000377971 [100%]
  ANKLE1-003 - ENSP00000377971 [100%]
 

Other gene match

    
ANTIGEN VIEW
ANKLE1-001
ANKLE1-003
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.