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Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
Antibody dilution
1:130
Literature conformity
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA016816
Antibody HPA017382
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA017382 across 75 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA016816 across 75 cell types.
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human fallopian tube shows strong positivity in cilia of glandular cells.
Immunohistochemical staining of human testis shows cytoplasmic positivity in spermatocytes and spermatides.
Expression
RNA: detected in 26 tissues Protein: detected in 12 cell types
RNA: detected in 26 tissues Protein: detected in 15 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:100
1:150
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA016816
Antibody HPA017382
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY409012)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
35.1, 30.8
35.1, 30.8
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA016816
Antibody HPA017382
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
