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G6PD

ANTIBODY INFORMATION
 
 

Antibody HPA000247

  

Antibody HPA000834

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA000247   HPA000834  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 1   1  

References

 2   3  

VALIDATION SUMMARY
 
 

Antibody HPA000247

  

Antibody HPA000834

  
Immunocytochemistry

Approved   Approved  
Immunohistochemistry


Validated
  

Validated
  
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA000247

  

Antibody HPA000834

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.   Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.  

Antibody dilution

 1:20   1:185  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA000247

  

Antibody HPA000834

  

Formal validation: Orthogonal

  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 

 

  

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		    

 

 

    

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 36 tissues.    
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human bone marrow shows strong positivity in subsets of hematopoietic cells.   Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferous ducts.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 62 cell types		   RNA: detected in 37 tissues
Protein: detected in 32 cell types		  

Retrieval

 HIER pH9   HIER pH6  

Antibody dilution

 1:200   1:4000  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA000247

  

Antibody HPA000834

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 63.8, 62.5, 59.3   63.8, 62.5, 59.3, 38.5, 36.5, 29.5  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA000247

  

Antibody HPA000834

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:2000   1:1000  

ANTIGEN INFORMATION
 
 

Antibody HPA000247

  

Antibody HPA000834

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 110   109  

Antigen sequence

 
EVRLQFHDVAGDIFHQQCKRNELVIRVQPNEAVYTKMMTKKPGMFFNPEE
SELDLTYGNRYKNVKLPDAYERLILDVFCGSQMHFVRSDELREAWRIFTP
LLHQIELEKP
  
FQGDAFHQSDTHIFIIMGASGDLAKKKIYPTIWWLFRDGLLPENTFIVGY
ARSRLTVADIRKQSEPFFKATPEEKLKLEDFFARNSYVAGQYDDAASYQR
LNSHMNALH
 

Matching transcripts

 G6PD-001 - ENSP00000377192 [100%]
G6PD-006 - ENSP00000377194 [100%]
G6PD-010 - ENSP00000358633 [100%]
G6PD-201 - ENSP00000483686 [100%]
  G6PD-001 - ENSP00000377192 [100%]
G6PD-002 - ENSP00000395599 [100%]
G6PD-005 - ENSP00000394690 [100%]
G6PD-006 - ENSP00000377194 [100%]
G6PD-007 - ENSP00000400648 [100%]
G6PD-010 - ENSP00000358633 [100%]
G6PD-201 - ENSP00000483686 [100%]
 

Other gene match

    
ANTIGEN VIEW
G6PD-001
G6PD-002
G6PD-005
G6PD-006
G6PD-007
G6PD-010
G6PD-201

RELEVANT PUBLICATIONS
 
 

Antibody HPA000247

  

Antibody HPA000834

  

1.

 Human Protein Atlas of redox systems - what can be learnt?
Dammeyer P et al
Biochim Biophys Acta 2011;1810(1):111-38
   Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model
Kato BS et al
Proteome Sci 2011;9:73
  

2.

 Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C et al
Nat Methods 2013;10(4):315-23
   TAp73 enhances the pentose phosphate pathway and supports cell proliferation
Du W et al
Nat Cell Biol 2013;15(8):991-1000
  

3.

   Global variability in gene expression and alternative splicing is modulated by mitochondrial content
Guantes R et al
Genome Res 2015;25(5):633-44
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.