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ANTIBODY INFORMATION
 
 

Antibody HPA032060

  

Antibody HPA032062

  

Antibody CAB034116

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA032060   HPA032062   sc-83295  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 7   12   12  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA032060

  

Antibody HPA032062

  

Antibody CAB034116

  
Immunocytochemistry

  Uncertain    
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA032060

  

Antibody HPA032062

  

Antibody CAB034116

  

Standard validation

   Uncertain    

     

Figure description

   Immunofluorescent staining of human cell line CACO-2 shows localization to cytosol.    

Antibody dilution

   1:45    

Literature conformity

   The subcellular location is not consistent with literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA032060

  

Antibody HPA032062

  

Antibody CAB034116

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 59 cell types		   RNA: detected in 37 tissues
Protein: detected in 69 cell types		   RNA: detected in 37 tissues
Protein: detected in 68 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:300   1:225   1:100  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA032060

  

Antibody HPA032062

  

Antibody CAB034116

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 83.3, 82.2, 74.2   83.3, 82.2, 29.6   83.3, 82.2, 74.2, 29.6, 15.3  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA032060

  

Antibody HPA032062

  

Antibody CAB034116

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA032060

  

Antibody HPA032062

  

Antibody CAB034116

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 141   83    

Antigen sequence

 
IQALLEAGGHHRPEFSSCLQKAHEFLRLSQVPDNPPDYQKYYRQMRKGGF
SFSTLDCGWIVSDCTAEALKAVLLLQEKCPHVTEHIPRERLCDAVAVLLN
MRNPDGGFATYETKRGGHLLELLNPSEVFGDIMIDYTYVEC
  
MTEGTCLRRRGGPYKTEPATDLGRWRLNCERGRQTWTYLQDERAGREQTG
LEAYALGLDTKNYFKDLPKAHTAFEGALNGMTF
  

											 
																				

																		

										
											
Matching transcripts

												
																					LSS-001 - ENSP00000380837 [100%] 
LSS-002 - ENSP00000348762 [100%] 
LSS-010 - ENSP00000429133 [100%] 
LSS-201 - ENSP00000409191 [100%] 

											 
																						LSS-001 - ENSP00000380837 [100%] 
LSS-002 - ENSP00000348762 [100%] 
LSS-010 - ENSP00000429133 [100%] 
LSS-005 - ENSP00000391368 [94%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
LSS-001
LSS-002
LSS-005
LSS-010
LSS-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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