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AMICA1

ANTIBODY INFORMATION
 
 

Antibody HPA047919

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA047919  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 10  

References

  

VALIDATION SUMMARY
 
 

Antibody HPA047919

  
Immunohistochemistry

Approved  
Western blot

Supported  
Protein array

Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA047919

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human rectum shows moderate cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 33 tissues
Protein: detected in 38 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:350  

Literature conformity

 Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA047919

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 44.3, 43.1, 43, 40.1  

Antibody dilution

 1:520  

PROTEIN ARRAY
 
 

Antibody HPA047919

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:4200  

ANTIGEN INFORMATION
 
 

Antibody HPA047919

  

Antigen

 Recombinant protein fragment  

Length (aa)

 83  

Antigen sequence

 
KKTCGNKSSVNSTVLVKNTKKTNPEIKEKPCHFERCEGEKHIYSPIIVRE
VIEEEEPSEKSEATYMTMHPVWPSLRSDRNNSL
 

Matching transcripts

 AMICA1-001 - ENSP00000348635 [100%]
AMICA1-002 - ENSP00000431218 [100%]
AMICA1-003 - ENSP00000292067 [100%]
AMICA1-004 - ENSP00000436117 [100%]
 

Other gene match

  
ANTIGEN VIEW
AMICA1-001
AMICA1-002
AMICA1-003
AMICA1-004
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.