Fields »
ABOUT
HELP
BLOG
CYP11B1

ANTIBODY INFORMATION
 
 

Antibody HPA049171

  

Antibody HPA056348

  

Antibody HPA057752

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA049171   HPA056348   HPA057752  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 CYP11B2 - ENSG00000179142 [100%]
   CYP11B2 - ENSG00000179142 [100%]
   CYP11B2 - ENSG00000179142 [100%]
  

Released in version

 10   14   14  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA049171

  

Antibody HPA056348

  

Antibody HPA057752

  
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA049171

  

Antibody HPA056348

  

Antibody HPA057752

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of adrenal gland shows strong cytoplasmic positivity in cortical cells.   Immunohistochemical staining of adrenal gland shows strong cytoplasmic positivity in cortical cells.   Immunohistochemical staining of adrenal gland shows moderate positivity in cortical cells.  

Expression

 RNA: detected in 3 tissues
Protein: detected in 3 cell types		   RNA: detected in 3 tissues
Protein: detected in 9 cell types		   RNA: detected in 3 tissues
Protein: detected in 5 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:1500   1:150   1:50  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA049171

  

Antibody HPA056348

  

Antibody HPA057752

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

      

Target mass (kDa)

 65.1, 57.6, 20.5   65.1, 57.6, 49.7   65.1, 57.6, 49.7  

Antibody dilution

 1:440   1:90   1:80  

PROTEIN ARRAY
 
 

Antibody HPA049171

  

Antibody HPA056348

  

Antibody HPA057752

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3500   1:500   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA049171

  

Antibody HPA056348

  

Antibody HPA057752

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 46   87   39  

Antigen sequence

 
FLYSLGRNAALFPRPERYNPQRWLDIRGSGRNFHHVPFGFGMRQCL
  
RFLPMVDAVARDFSQALKKKVLQNARGSLTLDVQPSIFHYTIEASNLALF
GERLGLVGHSPSSASLNFLHALEVMFKSTVQLMFMPR
  
VQQILRQESLAAAASISEHPQKATTELPLLRAALKETLR
 

Matching transcripts

 CYP11B1-001 - ENSP00000292427 [96%]
CYP11B1-003 - ENSP00000366903 [96%]
CYP11B1-005 - ENSP00000430144 [96%]
  CYP11B1-001 - ENSP00000292427 [100%]
CYP11B1-002 - ENSP00000428043 [100%]
CYP11B1-003 - ENSP00000366903 [100%]
  CYP11B1-001 - ENSP00000292427 [97%]
CYP11B1-002 - ENSP00000428043 [97%]
CYP11B1-003 - ENSP00000366903 [97%]
 

Other gene match

 CYP11B2 - ENSG00000179142 [100%]
   CYP11B2 - ENSG00000179142 [100%]
   CYP11B2 - ENSG00000179142 [100%]
  
ANTIGEN VIEW
CYP11B1-001
CYP11B1-002
CYP11B1-003
CYP11B1-005
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.