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ANTIBODY INFORMATION
 
 

Antibody HPA058265

  

Antibody CAB046486

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   SIGMA
  

Product name

 HPA058265   SAB4200008  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 13   10  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA058265

  

Antibody CAB046486

  
Immunocytochemistry

Approved    
Immunohistochemistry

Approved   Approved  
Western blot

Supported   Supported  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA058265

  

Antibody CAB046486

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line CACO-2 shows localization to centrosome & midbody ring.    

Antibody dilution

 1:68    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA058265

  

Antibody CAB046486

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.   Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 61 cell types		   RNA: detected in 37 tissues
Protein: detected in 79 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:300   1:500  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA058265

  

Antibody CAB046486

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 78.1, 76.3, 24.3   78.1, 76.3, 24.3  

Antibody dilution

 1:230   1:500  

PROTEIN ARRAY
 
 

Antibody HPA058265

  

Antibody CAB046486

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2700    

ANTIGEN INFORMATION
 
 

Antibody HPA058265

  

Antibody CAB046486

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 55    

Antigen sequence

 
SFHPHAGGGSQENLDNDTETDSLVSAQRERPRRRDGPEHATRLNGTAKGE
RRREP
   

Matching transcripts

 DVL3-001 - ENSP00000316054 [100%]
DVL3-002 - ENSP00000405885 [100%]
DVL3-005 - ENSP00000393849 [100%]
   

Other gene match

    
ANTIGEN VIEW
DVL3-001
DVL3-002
DVL3-005
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.