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Antibody staining overlaps with antibody HPA061642.
Validated
Antibody staining overlaps with antibody HPA017757.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line Hep G2 shows localization to nuclear speckles.
Immunofluorescent staining of human cell line SH-SY5Y shows localization to nuclear speckles.
Antibody dilution
1:8
1:72
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA017757
Antibody HPA061642
Standard validation
Approved
Figure description
Immunohistochemical staining of human duodenum shows moderate slightly granular cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 39 cell types
Retrieval
HIER pH6
Antibody dilution
1:10
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA017757
Antibody HPA061642
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408741)
Target mass (kDa)
21.4, 13.7
25, 21.4
Antibody dilution
1:250
1:240
PROTEIN ARRAY
Antibody HPA017757
Antibody HPA061642
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
