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ZFPL1

ANTIBODY INFORMATION
 
 

Antibody HPA014909

  

Antibody HPA017347

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA014909   HPA017347  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 4   4  

References

 1    

VALIDATION SUMMARY
 
 

Antibody HPA014909

  

Antibody HPA017347

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry


Validated

Validated
  

Validated
  
Western blot


Validated
   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA014909

  

Antibody HPA017347

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA017347.		  
Validated

Antibody staining overlaps with antibody HPA014909.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.   Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.  

Antibody dilution

 1:118   1:71  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA014909

  

Antibody HPA017347

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    

 

 

    

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.    
 

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  

 

  
 

  

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA017347 across 45 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA014909 across 45 cell lines.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in granular pattern in exocrine glandular cells.   Immunohistochemical staining of human rectum shows cytoplasmic positivity with a granular pattern in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 76 cell types		   RNA: detected in 37 tissues
Protein: detected in 68 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:50  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA014909

  

Antibody HPA017347

  

Formal validation: Genetic
Validated

Downregulation visible in both siRNA lanes		    

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		    

Target mass (kDa)

 34.1    

Loading control

 Total protein image    

Antibody dilution

 1:258    
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402028)		  

Target mass (kDa)

 34.1   34.1, 22.7, 20.6, 18.9  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA014909

  

Antibody HPA017347

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA014909

  

Antibody HPA017347

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 120   108  

Antigen sequence

 
DEVVSPEPEPLNTSDFSDWSSFNASSTPGPEEVDSASAAPAFYSQAPRPP
ASPGRPEQHTVIHMGNPEPLTHAPRKVYDTRDDDRTPGLHGDCDDDKYRR
RPALGWLARLLRSRAGSRKR
  
KCPKRKVTNLFCFEHRVNVCEHCLVANHAKCIVQSYLQWLQDSDYNPNCR
LCNIPLASRETTRLVCYDLFHWACLNERAAQLPRNTAPAGYQCPSCNGPI
FPPTNLAG
 

Matching transcripts

 ZFPL1-001 - ENSP00000294258 [100%]
  ZFPL1-001 - ENSP00000294258 [100%]
ZFPL1-005 - ENSP00000434454 [100%]
ZFPL1-013 - ENSP00000437090 [100%]
ZFPL1-006 - ENSP00000432529 [94%]
 

Other gene match

    
ANTIGEN VIEW
ZFPL1-001
ZFPL1-005
ZFPL1-006
ZFPL1-013

RELEVANT PUBLICATIONS
 
 

Antibody HPA014909

  

Antibody HPA017347

  

1.

 Membrane trafficking. The specificity of vesicle traffic to the Golgi is encoded in the golgin coiled-coil proteins
Wong M et al
Science 2014;346(6209):1256898
Application: ICC-IF 		   
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.