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Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferus ducts.
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
Expression
RNA: detected in 5 tissues Protein: detected in 37 cell types
RNA: detected in 5 tissues Protein: detected in 60 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:350
1:250
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA035889
Antibody HPA035890
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407412)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407412)
Target mass (kDa)
47.6, 47.2
47.6, 47.2
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA035889
Antibody HPA035890
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
