We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
Expression
RNA: detected in 6 tissues Protein: detected in 18 cell types
RNA: detected in 6 tissues Protein: detected in 26 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:25
1:6000
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA003278
Antibody CAB022450
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400891)
Target mass (kDa)
39.9
39.9
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA003278
Antibody CAB022450
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
