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SMC6

ANTIBODY INFORMATION
 
 

Antibody HPA035057

  

Antibody HPA042733

  

Antibody CAB037186

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   SIGMA
  

Product name

 HPA035057   HPA042733   S7822  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 13   12   12  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA035057

  

Antibody HPA042733

  

Antibody CAB037186

  
Immunocytochemistry

Supported      
Immunohistochemistry

  Uncertain   Uncertain  
Western blot

Supported   Supported   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA035057

  

Antibody HPA042733

  

Antibody CAB037186

  

Standard validation

 Supported      

     

Figure description

 Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.      

Antibody dilution

 1:93      

Literature conformity

 The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA035057

  

Antibody HPA042733

  

Antibody CAB037186

  

Standard validation

   Uncertain   Uncertain  

     

Figure description

   Immunohistochemical staining of human colon shows moderate cytoplasmic and nuclear positivity in glandular cells.   Immunohistochemical staining of human stomach, lower shows strong nuclear and cytoplasmic positivity in glandular cells.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 44 cell types		   RNA: detected in 37 tissues
Protein: detected in 55 cell types		  

Retrieval

   HIER pH6   HIER pH6  

Antibody dilution

   1:170   1:300  

Literature conformity

   Not consistent with gene/protein characterization data.   Not consistent with gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA035057

  

Antibody HPA042733

  

Antibody CAB037186

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 126.3, 84.5   126.3, 84.5   126.3, 84.5, 51, 8.6, 6.5  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA035057

  

Antibody HPA042733

  

Antibody CAB037186

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA035057

  

Antibody HPA042733

  

Antibody CAB037186

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 90   86    

Antigen sequence

 
ADERVLQALMKRFYLPGTSRPPIIVSEFRNEIYDVRHRAAYHPDFPTVLT
ALEIDNAVVANSLIDMRGIETVLLIKNNSVARAVMQSQKP
  
KEEHGKIKREELDVKHALSYNQRQLKELKDSKTDRLKRFGPNVPALLEAI
DDAYRQGHFTYKPVGPLGACIHLRDPELALAIESCL
  

											 
																				

																		

										
											
Matching transcripts

												
																					SMC6-001 - ENSP00000323439 [100%] 
SMC6-002 - ENSP00000404092 [100%] 
SMC6-003 - ENSP00000408644 [100%] 
SMC6-006 - ENSP00000384539 [100%] 

											 
																						SMC6-001 - ENSP00000323439 [100%] 
SMC6-002 - ENSP00000404092 [100%] 
SMC6-003 - ENSP00000408644 [100%] 
SMC6-006 - ENSP00000384539 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
SMC6-001
SMC6-002
SMC6-003
SMC6-006
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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