We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line MCF7 shows localization to intermediate filaments.
Antibody dilution
1:6
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA048600
Antibody HPA053929
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human fallopian tube shows distinct positivity in cilia of glandular cells.
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 2 tissues Protein: detected in 46 cell types
RNA: detected in 2 tissues Protein: detected in 7 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:175
1:100
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA048600
Antibody HPA053929
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408173)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
50.6
50.6, 14.9
Antibody dilution
1:250
1:80
PROTEIN ARRAY
Antibody HPA048600
Antibody HPA053929
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
