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CTSS

ANTIBODY INFORMATION
 
 

Antibody HPA002988

  

Antibody CAB000460

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   AbD Serotec
  

Product name

 HPA002988   MCA2075  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

    

Released in version

 2   1  

References

 2    

VALIDATION SUMMARY
 
 

Antibody HPA002988

  

Antibody CAB000460

  
Immunocytochemistry

Supported    
Immunohistochemistry


Validated
   Supported  
Western blot

Supported   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA002988

  

Antibody CAB000460

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.    

Antibody dilution

 1:31    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002988

  

Antibody CAB000460

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    

 

 

    

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.    
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.   Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in subsets of cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 43 cell types		   RNA: detected in 37 tissues
Protein: detected in 31 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:50  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002988

  

Antibody CAB000460

  

Standard validation

 Supported

Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401317)		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 37.5, 31.6   37.5, 31.6  

Antibody dilution

 1:250   1:800  

PROTEIN ARRAY
 
 

Antibody HPA002988

  

Antibody CAB000460

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA002988

  

Antibody CAB000460

  

Antigen

 Recombinant protein fragment   Not known  

Length (aa)

 141    

Antigen sequence

 
NGGFMTTAFQYIIDNKGIDSDASYPYKAMDQKCQYDSKYRAATCSKYTEL
PYGREDVLKEAVANKGPVSVGVDARHPSFFLYRSGVYYEPSCTQNVNHGV
LVVGYGDLNGKEYWLVKNSWGHNFGEEGYIRMARNKGNHCG
  

											 
																				

																		

										
											
Matching transcripts

												
																					CTSS-001 - ENSP00000357981 [100%] 
CTSS-006 - ENSP00000408414 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CTSS-001
CTSS-006
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA002988

												
										 
																				
											
											
											
Antibody CAB000460

												
										 
																		

																	

										
											
1.

												
																						Proteomics analysis of Hodgkin lymphoma: identification of new players involved in the cross-talk between HRS cells and infiltrating lymphocytes

	Ma Y et al

	Blood 2008;111(4):2339-46

			
											 
																						
											 
																				

																		

										
											
2.

												
																						Transcriptional control of autophagy-lysosome function drives pancreatic cancer metabolism

	Perera RM et al

	Nature 2015;524(7565):361-5

			Application: IHC				
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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by the Knut & Alice Wallenberg foundation.