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IWS1

ANTIBODY INFORMATION
 
 

Antibody HPA035719

  

Antibody HPA061866

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA035719   HPA061866  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   13  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA035719

  

Antibody HPA061866

  
Immunocytochemistry

 

Validated
  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA035719

  

Antibody HPA061866

  

Formal validation: Tagged protein

  
Validated

Antibody staining overlaps with GFP tagged protein		  

   

Figure description

   Immunofluorescent staining of transgenic HeLa cells show antibody staining in nucleoplasm and GFP expression in nucleoplasm.  

Antibody dilution

   1:43  
 

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm.  

Antibody dilution

   1:43  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA035719

  

Antibody HPA061866

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human tonsil shows strong nuclear positivity in reaction center cells.   Immunohistochemical staining of human rectum shows moderate nuclear positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 69 cell types		   RNA: detected in 37 tissues
Protein: detected in 71 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:700   1:800  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA035719

  

Antibody HPA061866

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

    

Target mass (kDa)

 92   92  

Antibody dilution

 1:250   1:140  

PROTEIN ARRAY
 
 

Antibody HPA035719

  

Antibody HPA061866

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:1700  

ANTIGEN INFORMATION
 
 

Antibody HPA035719

  

Antibody HPA061866

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 96   83  

Antigen sequence

 
IFGESGDEEEEEFTGFNQEDLEEEKGETQVKEAEDSDSDDNIKRGKHMDF
LSDFEMMLQRKKSMSGKRRRNRDGGTFISDADDVVSAMIVKMNEAA
  
NEELPKPRISDSESEDPPRNQASDSENEELPKPRVSDSESEGPQKGPASD
SETEDASRHKQKPESDDDSDRENKGEDTEMQND
 

Matching transcripts

 IWS1-001 - ENSP00000295321 [100%]
  IWS1-001 - ENSP00000295321 [100%]
 

Other gene match

    
ANTIGEN VIEW
IWS1-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.