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Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
Antibody dilution
1:50
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA027409
Antibody HPA027453
Antibody HPA030058
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous.
Expression
RNA: detected in 37 tissues Protein: detected in 78 cell types
RNA: detected in 37 tissues Protein: detected in 76 cell types
RNA: detected in 37 tissues Protein: detected in 63 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:40
1:35
1:300
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA027409
Antibody HPA027453
Antibody HPA030058
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
144, 141.7
144, 141.7
144, 141.7
Antibody dilution
1:250
1:250
1:250
PROTEIN ARRAY
Antibody HPA027409
Antibody HPA027453
Antibody HPA030058
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
