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Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts and strong nuclear staining in leydig cells.
Immunohistochemical staining of human rectum shows moderate cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 11 tissues Protein: detected in 61 cell types
RNA: detected in 11 tissues Protein: detected in 71 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:100
1:25
Literature conformity
Not consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA035516
Antibody HPA035517
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
160.2, 120.3, 82.4
160.2, 120.3
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA035516
Antibody HPA035517
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
