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AIMP1

ANTIBODY INFORMATION
 
 

Antibody HPA018476

  

Antibody CAB017618

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA018476   sc-66879  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 4   4  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA018476

  

Antibody CAB017618

  
Immunocytochemistry


Validated
   Supported  
Immunohistochemistry

Supported   Supported  
Western blot


Validated
   Supported  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA018476

  

Antibody CAB017618

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by one siRNA.		    

 

Relative fluorescence intensity (RFI)

    

Figure description

 Cytoplasmic region of segmented cells in 10x-images    

Antibody dilution

 1:8    
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.   Immunofluorescent staining of human cell line A-431 shows localization to cytosol.  

Antibody dilution

 1:15   1:62  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA018476

  

Antibody CAB017618

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human hippocampus shows distinct cytoplasmic positivity in neuronal cells.   Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 67 cell types		   RNA: detected in 37 tissues
Protein: detected in 67 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:20   1:250  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA018476

  

Antibody CAB017618

  

Formal validation: Genetic
Validated

Downregulation visible in one of two siRNA lanes		    

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		    

Target mass (kDa)

 37, 34.4    

Loading control

 Total protein image    

Antibody dilution

 1:58    
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 37, 34.4   37, 34.4, 16.6  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA018476

  

Antibody CAB017618

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA018476

  

Antibody CAB017618

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 101    

Antigen sequence

 
CNLKPAKMRGVLSQAMVMCASSPEKIEILAPPNGSVPGDRITFDAFPGEP
DKELNPKKKIWEQIQPDLHTNDECVATYKGVPFEVKGKGVCRAQTMSNSG
I
  

											 
																				

																		

										
											
Matching transcripts

												
																					AIMP1-001 - ENSP00000378191 [100%] 
AIMP1-002 - ENSP00000350699 [100%] 
AIMP1-201 - ENSP00000405248 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

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