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Antibody staining overlaps with antibody HPA029703.
Validated
Antibody staining overlaps with antibody HPA029700.
Standard validation
Supported
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Antibody dilution
1:100
1:50
1:100
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA029700
Antibody HPA029701
Antibody HPA029702
Antibody HPA029703
Formal validation: Independent
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA029702 across 46 cell lines. Pearson correlation with HPA029701 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA029702 across 46 cell lines. Pearson correlation with HPA029700 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA029701 across 46 cell lines. Pearson correlation with HPA029700 across 46 cell lines.
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA029703 across 74 cell types. Spearman correlation with HPA029702 across 72 cell types. Spearman correlation with HPA029701 across 74 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA029700 across 74 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA029700 across 72 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA029700 across 74 cell types.
Standard validation
Supported
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in tubular cells.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in tubular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 41 cell types
RNA: detected in 37 tissues Protein: detected in 32 cell types
RNA: detected in 37 tissues Protein: detected in 49 cell types
RNA: detected in 37 tissues Protein: detected in 55 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:300
1:25
1:1600
1:250
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA029700
Antibody HPA029701
Antibody HPA029702
Antibody HPA029703
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
81.7, 81.2
81.7, 81.2, 34.6, 26
81.7, 81.2, 31.9
81.7, 81.2
Antibody dilution
1:250
1:250
1:250
1:250
PROTEIN ARRAY
Antibody HPA029700
Antibody HPA029701
Antibody HPA029702
Antibody HPA029703
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
