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Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.
Antibody dilution
1:200
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA039149
Antibody HPA061446
Antibody CAB034450
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts.
Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in subset of cells in seminiferus ducts.
Immunohistochemical staining of human testis shows strong nuclear and cytoplasmic positivity in cells in seminiferus tubules.
Expression
RNA: detected in 34 tissues Protein: detected in 26 cell types
RNA: detected in 34 tissues Protein: detected in 7 cell types
RNA: detected in 34 tissues Protein: detected in 8 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:3000
1:850
1:3000
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA039149
Antibody HPA061446
Antibody CAB034450
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
94.5, 92
94.5, 92
94.5, 92, 59.2
Antibody dilution
1:250
1:90
1:500
PROTEIN ARRAY
Antibody HPA039149
Antibody HPA061446
Antibody CAB034450
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.