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Immunofluorescent staining of human cell line HEK 293 shows localization to cytosol.
Antibody dilution
1:7
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA053752
Antibody HPA058032
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human kidney shows strong membranous positivity in renal tubules.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.
Expression
RNA: detected in 36 tissues Protein: detected in 6 cell types
RNA: detected in 36 tissues Protein: detected in 3 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:75
1:25
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA053752
Antibody HPA058032
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
72.9, 63.1, 55.5
72.9, 63.1
Antibody dilution
1:250
1:80
PROTEIN ARRAY
Antibody HPA053752
Antibody HPA058032
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
