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Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
Antibody dilution
1:87
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA056004
Antibody CAB010887
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human parathyroid gland shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human parathyroid gland shows moderate cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 36 tissues Protein: detected in 28 cell types
RNA: detected in 36 tissues Protein: detected in 62 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:15
1:350
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA056004
Antibody CAB010887
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
77.5, 70.2
77.5, 70.2, 48.5, 16.1
Antibody dilution
1:90
1:500
PROTEIN ARRAY
Antibody HPA056004
Antibody CAB010887
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
