MIOS - Antibodies - The Human Protein Atlas

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MIOS

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA042928

Antibody HPA052223

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA042928

HPA052223

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA042928

Antibody HPA052223

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA042928

Antibody HPA052223

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA052223.

Validated

Antibody staining overlaps with antibody HPA042928.

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, cytosol & cell junctions.

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & cytosol.

Antibody dilution

1:77

1:55

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA042928

Antibody HPA052223

Standard validation

Approved

Figure description

Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 65 cell types

Retrieval

HIER pH6

Antibody dilution

1:500

Literature conformity

No avaliable gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA042928

Antibody HPA052223

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Target mass (kDa)

98.6

Antibody dilution

1:250

1:270

PROTEIN ARRAY

Antibody HPA042928

Antibody HPA052223

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:2200

ANTIGEN INFORMATION

Antibody HPA042928

Antibody HPA052223

Antigen

Recombinant protein fragment

Length (aa)

122

120

Antigen sequence

ACKFLSDTQLNRYIEKLTNEMKEAGNLEGILLTGLTKDGVDLMESYVDRT
GDVQTASYCMLQGSPLDVLKDERVQYWIENYRNLLDAWRFWHKRAEFDIH
RSKLDPSSKPLAQVFVSCNFCG

SISYSCSAVPHQGRGFSQYGVSGSPTKSKVTSCPGCRKPLPRCALCLINM
GTPVSSCPGGTKSDEKVDLSKDKKLAQFNNWFTWCHNCRHGGHAGHMLSW
FRDHAECPVSACTCKCMQLD

Matching transcripts

MIOS-001 - ENSP00000339881 [100%]
MIOS-002 - ENSP00000384088 [100%]

Other gene match

ANTIGEN VIEW

MIOS-001	MIOS-002

RELEVANT PUBLICATIONS

Antibody HPA042928

Antibody HPA052223

Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C et al
Nat Methods 2013;10(4):315-23

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.