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FNDC1

ANTIBODY INFORMATION
 
 

Antibody HPA030962

  

Antibody HPA030963

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA030962   HPA030963  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   12  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA030962

  

Antibody HPA030963

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA030962

  

Antibody HPA030963

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA030963.		  
Validated

Antibody staining overlaps with antibody HPA030962.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.   Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.  

Antibody dilution

 1:141   1:105  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA030962

  

Antibody HPA030963

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity(granular pattern) in neuronal cells.   Immunohistochemical staining of human cerebral cortex shows strong nuclear membranous positivity in neuronal cells.  

Expression

 RNA: detected in 21 tissues
Protein: detected in 51 cell types		   RNA: detected in 21 tissues
Protein: detected in 48 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:300   1:75  

Literature conformity

 Not consistent with gene/protein characterization data.   Not consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA030962

  

Antibody HPA030963

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

    

Target mass (kDa)

 205.6, 194.4   205.6, 194.4  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA030962

  

Antibody HPA030963

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA030962

  

Antibody HPA030963

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 81   99  

Antigen sequence

 
DSHPKLSSGIHGDEEDEKPLPATVVNDHVPSSSRQPISRGWEDLRRSPQR
GASLHRKEPIPENPKSTGADTHPQGKYSSLA
  
ECYAEEDEFSGLETDTAVPTEEAYVIYDEDYEFETSRPPTTTEPSTTATT
PRVIPEEGAISSFPEEEFDLAGRKRFVAPYVTYLNKDPSAPCSLTDALD
 

Matching transcripts

 FNDC1-001 - ENSP00000297267 [100%]
FNDC1-002 - ENSP00000333297 [100%]
  FNDC1-001 - ENSP00000297267 [100%]
FNDC1-002 - ENSP00000333297 [100%]
 

Other gene match

    
ANTIGEN VIEW
FNDC1-001
FNDC1-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.