Cell atlas - RAD21 - The Human Protein Atlas

GENERAL INFORMATION
Gene name	RAD21
Gene description	RAD21 cohesin complex component
Protein class	Cancer-related genes Disease related genes Predicted intracellular proteins
Predicted localization	Intracellular
Number of transcripts	8
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HUMAN PROTEIN ATLAS INFORMATION
Summary	Localized to the nucleoplasm.
RNA cell category	Expressed in all
Protein evidence	Evidence at protein level
Main location	Localized to the Nucleoplasm (validated)
siRNA	The location(s) are Validated based on siRNA mediated gene silencing.

DATA RELIABILITY
Reliability score	Validated based on 2 antibodies. HPA020044 , CAB022065
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ASSAYS

RNA expression

Human cells

siRNA

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all Organ Origin Category Expression Alphabetical Cell lines sorted after organ of phenotypic resemblance. Cell lines sorted after biological source for establishment. Cell lines sorted after the cell line category according to Cellosaurus. Cell lines sorted on descending RNA expression. Cell lines sorted alphabetically.

HUMAN CELLS

Summary

Localized to the nucleoplasm.

Main location

Nucleoplasm (validated)


	Toggle channels	Low High G1 S G2 M N/A

Antibody	Cell line	Location
HPA020044	A-431	Nucleoplasm
HPA020044	U-2 OS	Nucleoplasm
HPA020044	U-251 MG	Nucleoplasm
CAB022065	A-431	Nucleoplasm
CAB022065	U-2 OS	Nucleoplasm
CAB022065	U-251 MG	Nucleoplasm

siRNA VALIDATION

Relative fluorescence intensity (RFI)

Antibody

HPA020044

siRNA validation

Validated: Significant downregulation > 25% by one siRNA.

Analysis performed in

Nuclear region of segmented cells in 10x-images

siRNA 2

RFI

19%

p-value

< 0.01


	Toggle channels	Low High G1 S G2 M N/A

Thumbnail 10x	Thumbnail 40x	Antibody	Sample	Cell line
		HPA020044	siRNA 2	U-2 OS
		HPA020044	scrambled	U-2 OS

GENE INFORMATION
Gene name	RAD21 (HGNC Symbol)
Synonyms	hHR21, KIAA0078, SCC1
Description	RAD21 cohesin complex component (HGNC Symbol)
Entrez gene summary	The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq, Jul 2008]
Chromosome	8
Cytoband	q24.11
Chromosome location (bp)	116845935 - 116874866
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000164754 (version 83.38)
Entrez gene	5885
UniProt	O60216 (UniProt - Evidence at protein level)
neXtProt	NX_O60216
Antibodypedia	RAD21 antibodies

PROTEIN BROWSER

RAD21-001	RAD21-003	RAD21-004	RAD21-005	RAD21-006	RAD21-009	RAD21-010	RAD21-011

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
RAD21-001 ENSP00000297338 ENST00000297338	O60216 [Direct mapping] Double-strand-break repair protein rad21 homolog A0A024R9J0 [Target identity:100%; Query identity:100%] RAD21 homolog (S. pombe), isoform CRA_a Show all	Predicted intracellular proteins Cancer-related genes Mutated cancer genes Mutational cancer driver genes COSMIC somatic mutations in cancer genes COSMIC Somatic Mutations COSMIC Nonsense Mutations COSMIC Missense Mutations COSMIC Frameshift Mutations Disease related genes Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000228 [nuclear chromosome] GO:0000278 [mitotic cell cycle] GO:0000775 [chromosome, centromeric region] GO:0000785 [chromatin] GO:0000794 [condensed nuclear chromosome] GO:0001228 [transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding] GO:0005515 [protein binding] GO:0005654 [nucleoplasm] GO:0005694 [chromosome] GO:0005829 [cytosol] GO:0006302 [double-strand break repair] GO:0006310 [DNA recombination] GO:0006357 [regulation of transcription from RNA polymerase II promoter] GO:0006366 [transcription from RNA polymerase II promoter] GO:0006915 [apoptotic process] GO:0007059 [chromosome segregation] GO:0007067 [mitotic nuclear division] GO:0007131 [reciprocal meiotic recombination] GO:0008278 [cohesin complex] GO:0016020 [membrane] GO:0016925 [protein sumoylation] GO:0034991 [nuclear meiotic cohesin complex] GO:0043687 [post-translational protein modification] GO:0044267 [cellular protein metabolic process] GO:0045944 [positive regulation of transcription from RNA polymerase II promoter] GO:0051301 [cell division] GO:0071168 [protein localization to chromatin] Show all	631 aa 71.7 kDa	No	0
RAD21-003 ENSP00000428513 ENST00000523986	E5RI01 [Direct mapping] Double-strand-break repair protein rad21 homolog Show all	Predicted intracellular proteins Cancer-related genes Mutated cancer genes Mutational cancer driver genes COSMIC somatic mutations in cancer genes COSMIC Somatic Mutations COSMIC Nonsense Mutations COSMIC Missense Mutations COSMIC Frameshift Mutations Protein evidence (Ezkurdia et al 2014) Show all	GO:0000228 [nuclear chromosome] Show all	135 aa 15.6 kDa	No	0
RAD21-004 ENSP00000428158 ENST00000522699	E5RJW1 [Direct mapping] Double-strand-break repair protein rad21 homolog Show all	Predicted intracellular proteins Cancer-related genes Mutated cancer genes Mutational cancer driver genes COSMIC somatic mutations in cancer genes COSMIC Somatic Mutations COSMIC Nonsense Mutations COSMIC Missense Mutations COSMIC Frameshift Mutations Protein evidence (Ezkurdia et al 2014) Show all	GO:0005515 [protein binding] Show all	120 aa 13.8 kDa	No	0
RAD21-005 ENSP00000427923 ENST00000517485	E5RFV8 [Direct mapping] Double-strand-break repair protein rad21 homolog Show all	Predicted intracellular proteins Cancer-related genes Mutated cancer genes Mutational cancer driver genes COSMIC somatic mutations in cancer genes COSMIC Somatic Mutations COSMIC Nonsense Mutations COSMIC Missense Mutations COSMIC Frameshift Mutations Protein evidence (Ezkurdia et al 2014) Show all	GO:0005515 [protein binding] Show all	225 aa 25.8 kDa	No	0
RAD21-006 ENSP00000429342 ENST00000520992	E5RIN7 [Direct mapping] Double-strand-break repair protein rad21 homolog Show all	Predicted intracellular proteins Cancer-related genes Mutated cancer genes Mutational cancer driver genes COSMIC somatic mutations in cancer genes COSMIC Somatic Mutations COSMIC Nonsense Mutations COSMIC Missense Mutations COSMIC Frameshift Mutations Protein evidence (Ezkurdia et al 2014) Show all	GO:0005515 [protein binding] Show all	228 aa 26 kDa	No	0
RAD21-009 ENSP00000430273 ENST00000517749	E5RG18 [Direct mapping] Double-strand-break repair protein rad21 homolog Show all	Predicted intracellular proteins Cancer-related genes Mutated cancer genes Mutational cancer driver genes COSMIC somatic mutations in cancer genes COSMIC Somatic Mutations COSMIC Nonsense Mutations COSMIC Missense Mutations COSMIC Frameshift Mutations Protein evidence (Ezkurdia et al 2014) Show all	GO:0000228 [nuclear chromosome] Show all	69 aa 7.8 kDa	No	0
RAD21-010 ENSP00000428003 ENST00000518055	E5RFZ5 [Direct mapping] Double-strand-break repair protein rad21 homolog Show all	Predicted intracellular proteins Cancer-related genes Mutated cancer genes Mutational cancer driver genes COSMIC somatic mutations in cancer genes COSMIC Somatic Mutations COSMIC Nonsense Mutations COSMIC Missense Mutations COSMIC Frameshift Mutations Protein evidence (Ezkurdia et al 2014) Show all	GO:0000228 [nuclear chromosome] Show all	176 aa 20.1 kDa	No	0
RAD21-011 ENSP00000430524 ENST00000519837	E5RJK5 [Direct mapping] Double-strand-break repair protein rad21 homolog Show all	Predicted intracellular proteins Cancer-related genes Mutated cancer genes Mutational cancer driver genes COSMIC somatic mutations in cancer genes COSMIC Somatic Mutations COSMIC Nonsense Mutations COSMIC Missense Mutations COSMIC Frameshift Mutations Protein evidence (Ezkurdia et al 2014) Show all	GO:0005515 [protein binding] Show all	124 aa 14.2 kDa	No	0