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SNAPC3

ANTIBODY INFORMATION
 
 

Antibody HPA053145

  

Antibody HPA066031

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA053145   HPA066031  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 12   16  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA053145

  

Antibody HPA066031

  
Immunocytochemistry

Supported   Supported  
Western blot

Uncertain   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA053145

  

Antibody HPA066031

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nuclear bodies.   Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.  

Antibody dilution

 1:37   1:192  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

WESTERN BLOT
 
 

Antibody HPA053145

  

Antibody HPA066031

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 46.8   46.8, 27.1  

Antibody dilution

 1:180   1:640  

PROTEIN ARRAY
 
 

Antibody HPA053145

  

Antibody HPA066031

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1450   1:7700  

ANTIGEN INFORMATION
 
 

Antibody HPA053145

  

Antibody HPA066031

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 105   96  

Antigen sequence

 
FPYLYCHQGDCEHVIVITDIRLVHHDDCLDRTLYPLLIKKHWLWTRKCFV
CKMYTARWVTNNDSFAPEDPCFFCDVCFRMLHYDSEGNKLGEFLAYPYVD
PGTFN
  
LPELNTRAFHVGAFGELWRGRLRGAGDLSLREPPASALPGSQAADSDRED
AAVARDLDCSLEAAAELRAVCGLDKLKCLEDGEDPEVIPENTDLVT
 

Matching transcripts

 SNAPC3-002 - ENSP00000370200 [100%]
SNAPC3-201 - ENSP00000483273 [100%]
  SNAPC3-002 - ENSP00000370200 [100%]
SNAPC3-006 - ENSP00000391832 [100%]
SNAPC3-201 - ENSP00000483273 [100%]
 

Other gene match

    
ANTIGEN VIEW
SNAPC3-002
SNAPC3-006
SNAPC3-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.