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NSD1

ANTIBODY INFORMATION
 
 

Antibody HPA048431

  

Antibody HPA070333

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA048431   HPA070333  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 16   16  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA048431

  

Antibody HPA070333

  
Immunocytochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA048431

  

Antibody HPA070333

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line HEK 293 shows localization to nucleus & cytosol.   Immunofluorescent staining of human cell line HEK 293 shows localization to nucleus & plasma membrane.  

Antibody dilution

 1:70   1:30  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

WESTERN BLOT
 
 

Antibody HPA048431

  

Antibody HPA070333

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		  

   

Figure description

    

Target mass (kDa)

 296.7, 267.3   296.7, 267.3  

Antibody dilution

 1:350   1:100  

PROTEIN ARRAY
 
 

Antibody HPA048431

  

Antibody HPA070333

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:2800   1:1100  

ANTIGEN INFORMATION
 
 

Antibody HPA048431

  

Antibody HPA070333

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 131   81  

Antigen sequence

 
INEECSLKCCSSDTKGSPLASISKSGKVDGLKLLNNMHEKTRDSSDIETA
VVKHVLSELKELSYRSLGEDVSDSGTSKPSKPLLFSSASSQNHIPIEPDY
KFSTLLMMLKDMHDSKTKEQRLMTAQNLVSY
  
FSDVHFDSKVKQSDPGKISEKGLSFENGKGPELDSVMNSENDELNGVNQV
VPKKRWQRLNQRRTKPRKRMNRFKEKENSEC
 

Matching transcripts

 NSD1-001 - ENSP00000395929 [100%]
NSD1-002 - ENSP00000343209 [100%]
NSD1-201 - ENSP00000346111 [100%]
  NSD1-001 - ENSP00000395929 [100%]
NSD1-002 - ENSP00000343209 [100%]
NSD1-201 - ENSP00000346111 [100%]
 

Other gene match

    
ANTIGEN VIEW
NSD1-001
NSD1-002
NSD1-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.