Fields »
ABOUT
HELP
BLOG
DDX21

ANTIBODY INFORMATION
 
 

Antibody HPA036592

  

Antibody HPA036593

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA036592   HPA036593  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   12  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA036592

  

Antibody HPA036593

  
Immunocytochemistry


Validated
  

Validated

Validated
  
Immunohistochemistry


Validated
  

Validated
  
Western blot

Supported  

Validated
  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA036592

  

Antibody HPA036593

  

Formal validation: Genetic

  
Validated

Significant downregulation > 25% by one siRNA.		  

 
 

Relative fluorescence intensity (RFI)

  

Figure description

   Nuclear region of segmented cells in 10x-images  

Antibody dilution

   1:118  
 

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA036593.		  
Validated

Antibody staining overlaps with antibody HPA036592.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus & nucleoli.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli.  

Antibody dilution

 1:60   1:118  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA036592

  

Antibody HPA036593

  

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  

 

  
 

  

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA036593 across 74 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA036592 across 74 cell types.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human stomach shows strong nucleolar positivity in glandular cells.   Immunohistochemical staining of human vulva/anal skin shows strong nucleolar positivity in epidermal cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 51 cell types		   RNA: detected in 37 tissues
Protein: detected in 57 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:300   1:400  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA036592

  

Antibody HPA036593

  

Formal validation: Genetic

  
Validated

Downregulation visible in one of two siRNA lanes		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		  

Target mass (kDa)

   87.3, 79.7  

Loading control

   Total protein image  

Antibody dilution

   1:263  
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 87.3   87.3, 79.7  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA036592

  

Antibody HPA036593

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA036592

  

Antibody HPA036593

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 73   85  

Antigen sequence

 
KPKSDKTEEIAEEEETVFPKAKQVKKKAEPSEVDMNSPKSKKAKKKEEPS
QNDISPKTKSLRKKKEPIEKKVV
  
DSRRWQLSVATEQPELEGPREGYGGFRGQREGSRGFRGQRDGNRRFRGQR
EGSRGPRGQRSGGGNKSNRSQNKGQKRSFSKAFGQ
 

Matching transcripts

 DDX21-001 - ENSP00000346120 [100%]
  DDX21-001 - ENSP00000346120 [100%]
DDX21-201 - ENSP00000480334 [100%]
 

Other gene match

    
ANTIGEN VIEW
DDX21-001
DDX21-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.