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CEP295

ANTIBODY INFORMATION
 
 

Antibody HPA038595

  

Antibody HPA038596

  

Antibody HPA038597

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA038595   HPA038596   HPA038597  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 13   7   12  

References

   1    

VALIDATION SUMMARY
 
 

Antibody HPA038595

  

Antibody HPA038596

  

Antibody HPA038597

  
Immunocytochemistry

 

Validated
  

Validated
  
Immunohistochemistry


Validated
   Approved  

Validated
  
Western blot

Uncertain   Supported   Uncertain  
Protein array

Supported   Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA038595

  

Antibody HPA038596

  

Antibody HPA038597

  

Formal validation: Independent

  
Validated

Antibody staining overlaps with antibody HPA038597.		  
Validated

Antibody staining overlaps with antibody HPA038596.		  
 

Standard validation

   Approved   Approved  

     

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.   Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.  

Antibody dilution

   1:196   1:200  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA038595

  

Antibody HPA038596

  

Antibody HPA038597

  

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		    
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  

 

    
 

  

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA038597 across 72 cell types.		     Distribution of protein expression (antibody staining).
Spearman correlation with HPA038595 across 72 cell types.		  
 

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human testis shows strong cytoplasmic positivity in Leydig cells.   Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in seminiferous duct cells and Leydig cells.   Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 60 cell types		   RNA: detected in 37 tissues
Protein: detected in 73 cell types		   RNA: detected in 37 tissues
Protein: detected in 58 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:450   1:300   1:1000  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA038595

  

Antibody HPA038596

  

Antibody HPA038597

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		  

     

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 295.2   295.2, 87.7   295.2  

Antibody dilution

 1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA038595

  

Antibody HPA038596

  

Antibody HPA038597

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:12000   1:6000   1:6000  

ANTIGEN INFORMATION
 
 

Antibody HPA038595

  

Antibody HPA038596

  

Antibody HPA038597

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 98   102   101  

Antigen sequence

 
NTSSEQTGSSSFIPQLVQLSFTSLASAESGTILEPLFTESESKIFSSHLQ
IPQLQDRLLRISQLIQPQQDNLKALQEQLATQREAIILARQEAREELL
  
LRVSISREQSFFGSPLAHDPFSCLQLVGQENVCGDDYDEAVKLKESVVEN
HAVLSYAVEEEHAYLGPTVKPDDKAKTLSYEPLSSATVSTGSLLSYENTD
LS
  
VISVPSWKSERPTAISEHWDQGQRLKLSPNKYQPIQPIQTSKLEQDHFQV
ARQNHFPQRQVETTETLRASDILTNQALESQEHLRQFSQTETQQRDYKLV
P
 

Matching transcripts

 CEP295-001 - ENSP00000316681 [100%]
  CEP295-001 - ENSP00000316681 [100%]
CEP295-002 - ENSP00000437323 [100%]
  CEP295-001 - ENSP00000316681 [100%]
 

Other gene match

      
ANTIGEN VIEW
CEP295-001
CEP295-002

RELEVANT PUBLICATIONS
 
 

Antibody HPA038595

  

Antibody HPA038596

  

Antibody HPA038597

  

1.

   Cep295 is a conserved scaffold protein required for generation of a bona fide mother centriole
Tsuchiya Y et al
Nat Commun 2016;7:12567
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.