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Immunohistochemical staining of human cerebral cortex shows neuropil positivity.
Expression
RNA: detected in 16 tissues Protein: detected in 17 cell types
Retrieval
HIER pH6
Antibody dilution
1:200
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA052338
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408569)
Target mass (kDa)
24.7, 17.4, 14.2, 13.2, 11.5, 8.3
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA052338
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
