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FBN1

ANTIBODY INFORMATION
 
 

Antibody HPA017759

  

Antibody HPA021057

  

Antibody CAB002670

  

Antibody CAB058696

  

Antibody CAB068188

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Lab Vision/NeoMarkers
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA017759   HPA021057   MS-232   AMAb90584   AMAb90585  

Host species

 Rabbit   Rabbit   Mouse   Mouse   Mouse  

Clonality

 pAb   pAb   mAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known   Protein A/G   Protein A/G  

Other gene match

          

Released in version

 4   5   1   11   13  

References

   3        

VALIDATION SUMMARY
 
 

Antibody HPA017759

  

Antibody HPA021057

  

Antibody CAB002670

  

Antibody CAB058696

  

Antibody CAB068188

  
Immunocytochemistry

  Approved        
Immunohistochemistry

Supported  

Validated
   Supported  

Validated
  

Validated
  
Western blot

Uncertain   Uncertain   Uncertain   Uncertain   Uncertain  
Protein array

Supported   Approved        

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA017759

  

Antibody HPA021057

  

Antibody CAB002670

  

Antibody CAB058696

  

Antibody CAB068188

  

Standard validation

   Approved        

         

Figure description

   Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.        

Antibody dilution

   1:100        

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.        

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA017759

  

Antibody HPA021057

  

Antibody CAB002670

  

Antibody CAB058696

  

Antibody CAB068188

  

Formal validation: Orthogonal

  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 

 

    
 

 

  
 

 

  

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported   Supported   Supported   Supported   Supported  

         

Figure description

 Immunohistochemical staining of human bronchus shows moderate cytoplasmic positivity in respiratory epithelial cells and strong positivity in collagen fibers.   Immunohistochemical staining of human ovary shows distinct positivity in ovarian stroma cells.   Immunohistochemical staining of human skin shows distinct positivity in collagen fibers.   Immunohistochemical staining of human uterus, post-menopause shows strong cytoplasmic positivity in cells in endometrial stroma.   Immunohistochemical staining of human uterus shows strong positivity in stromal cells.  

Expression

 RNA: detected in 35 tissues
Protein: detected in 40 cell types		   RNA: detected in 35 tissues
Protein: detected in 13 cell types		   RNA: detected in 35 tissues
Protein: detected in 7 cell types		   RNA: detected in 35 tissues
Protein: detected in 9 cell types		   RNA: detected in 35 tissues
Protein: detected in 11 cell types		  

Retrieval

 HIER pH6   HIER pH6   None   HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:1000   1:50   1:250   1:100  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA017759

  

Antibody HPA021057

  

Antibody CAB002670

  

Antibody CAB058696

  

Antibody CAB068188

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		  

         

Figure description

       Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 312.3   312.3   312.3, 5.8   312.3, 5.8   312.3, 5.8  

Antibody dilution

 1:250   1:250   1:500   1:500   1:1000  

PROTEIN ARRAY
 
 

Antibody HPA017759

  

Antibody HPA021057

  

Antibody CAB002670

  

Antibody CAB058696

  

Antibody CAB068188

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		        

         

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.        

Antibody dilution

 1:3000   1:500        

ANTIGEN INFORMATION
 
 

Antibody HPA017759

  

Antibody HPA021057

  

Antibody CAB002670

  

Antibody CAB058696

  

Antibody CAB068188

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known   Recombinant protein   Recombinant protein  

Length (aa)

 149   147        

Antigen sequence

 
HCVSGMGMGRGNPEPPVSGEMDDNSLSPEACYECKINGYPKRGRKRRSTN
ETDASNIEDQSETEANVSLASWDVEKTAIFAFNISHVSNKVRILELLPAL
TTLTNHNRYLIESGNEDGFFKINQKEGISYLHFTKKKPVAGTYSLQISS
  
DVRPGYCYTALTNGRCSNQLPQSITKMQCCCDAGRCWSPGVTVAPEMCPI
RATEDFNKLCSVPMVIPGRPEYPPPPLGPIPPVLPVPPGFPPGPQIPVPR
PPVEYLYPSREPPRVLPVNVTDYCQLVRYLCQNGRCIPTPGSCRCEC
  

											 
																						

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					FBN1-001 - ENSP00000325527 [100%] 

											 
																						FBN1-001 - ENSP00000325527 [99%] 

											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
FBN1-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA017759

												
										 
																				
											
											
											
Antibody HPA021057

												
										 
																				
											
											
											
Antibody CAB002670

												
										 
																				
											
											
											
Antibody CAB058696

												
										 
																				
											
											
											
Antibody CAB068188

												
										 
																		

																	

										
											
1.

												
																					
											 
																							CD99 is a novel prognostic stromal marker in non-small cell lung cancer

	Edlund K et al

	Int J Cancer 2012;131(10):2264-73

			
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																					
											 
																							Network-based survival analysis reveals subnetwork signatures for predicting outcomes of ovarian cancer treatment

	Zhang W et al

	PLoS Comput Biol 2013;9(3):e1002975

			
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
3.

												
																					
											 
																							Epithelial-mesenchymal status influences how cells deposit fibrillin microfibrils

	Baldwin AK et al

	J Cell Sci 2014;127(Pt 1):158-71

			
											 
																						
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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