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Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies.
Antibody dilution
1:92
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA054967
Antibody HPA066900
Standard validation
Approved
Figure description
Immunohistochemical staining of human placenta shows moderate nucleolar positivity in decidual cells.
Expression
RNA: detected in 37 tissues Protein: detected in 53 cell types
Retrieval
HIER pH6
Antibody dilution
1:800
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA054967
Antibody HPA066900
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
88.3
88.3, 72.3
Antibody dilution
1:460
1:320
PROTEIN ARRAY
Antibody HPA054967
Antibody HPA066900
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
